T载体
pUC-T Easy Vector Systems
INSTRUCTIONS FOR USE OF PRODUCTS T1101
and 900μl to the uncut DNA control tube. Incubate for 1.5 hours at 37°C with shaking (~150rpm) .
7. Plate 100μl of each transformation culture onto duplicate LB/ampicillin/IPTG/X-Gal plates. For the uncut DNA control, a 1:10 dilution with SOC is recommended.
8. Incubate plates overnight at 37°C. Select white colonies.
5
CAGCACTGACCCTTTTGGGACCGC
BcaBestTM Sequencing Primer M13-47
，请把【付款记录详情】截图给客服，同时把您购买的文章【网址】发给客服。客服会在24小时内把文档发送给您。
