AP214 ameliorates sepsis-induced acute kidney injury and mor(7)

was conducted (EnVison+ System-HRP (DAB), DakoCytomation, Glostrup, Denmark) with

diaminobenzidine substrate, then counterstaining with hematoxylin. Positive stained cells were

counted from five randomly selected non-overlapping 200X fields.

Statistical analysis

Results of statistical analyses are expressed as means ± SEM. Differences among experimental

groups were confirmed by one-way ANOVA followed by Dunnett's test for individual

comparison of group means vs the control group. Survival analyses were compared by a log-

rank test. These calculations were performed with SigmaStat v3.10 (Systat Software Inc,

Richmond, CA). The null hypothesis was rejected when P < 0.05.NIH-PA Author Manuscript

NIH-PA Author Manuscript

NIH-PA Author ManuscriptSupplementary MaterialRefer to Web version on PubMed Central for supplementary material.AcknowledgementsWe thank Dr. Yan Qin for technical assistance.Disclosure This research was supported by the Intramural Research Program of the NIH, NIDDK. Thomas Jonassen,Jørgen Frøkiær, and Søren Nielsen have a financial interest in Action Pharma, which supplied no research funding,other than providing AP214.References1. Martin GS, Mannino DM, Eaton S, et al. The epidemiology of sepsis in the United States from 1979through 2000. N Engl J Med 2003;348:1546–1554. [PubMed: 12700374]2. Levy EM, Viscoli CM, Horwitz RI. The effect of acute renal failure on mortality. A cohort analysis.Jama 1996;275:1489–1494. [PubMed: 8622223]3. Neveu H, Kleinknecht D, Brivet F, et al. Prognostic factors in acute renal failure due to sepsis. Results

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