lipofectamineLTX_and_PLUS_man

Lipofectamine®

LTX & Plus Reagent

*Plus Reagent (Cat. no. 11514-015) available separately.

Pub. Part No. 15338.pps

Pub. No. MAN0001225

Rev. Date 20 July 2012

Description

Lipofectamine® LTX and Plus Reagent are proprietary formulations for

transfecting nucleic acids into a wide range of eukaryotic cells. Nucleic acid-Lipofectamine® LTX complexes can be added directly to

cells in culture medium, in the presence or absence of serum/antibiotic.

It is not necessary to remove complexes or change/add medium after transfection.

Important Guidelines for Transfection

Use Opti-MEM® I Reduced Serum Medium (Cat. no. 31985-062) to

dilute Lipofectamine® LTX Reagent and nucleic acids.

The amount of Lipofectamine® LTX Reagent required for successful transfection varies depending on the cell type and passage number. Start any new transfection by testing the recommended four concentrations of Lipofectamine® LTX Reagent to determine an optimum amount.

For additional information, and protocols on transfecting suspension cells refer to the manual at /transfection

For research use only. Not for human or animal therapeutic or diagnostic use.