槲皮素干预实验性慢性肾功能衰竭大鼠肾脏组织

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成都中医药大学学报

JournalofChengduUniversityofTCM2007年6月第30卷第2期Jun12007,Vol130,No12

槲皮素干预实验性慢性肾功能衰竭

大鼠肾脏组织病理改变

王天然　周纬　曾祥元　胡晓莉　邹自英　陈莉

(成都军区总医院　　)

　　摘　要:目的:。方法:给实,2;干预组口服槲皮素(100mg/Kg d×42d)治疗;;烘干法测定肾脏组织含水量;常规方法对肾脏行病理学检查;测定羟脯氨酸以观察肾组织纤维化程度;免疫组织化学染色法观察肾组织NF-κB变化。结果:与肾衰对照组比较,干预组大鼠肾脏指数和肾组织含水量均显著降低(5108%±0189%和78124%±1125%vs12163%±2194%和79129%±0190%),肾脏病理学损伤改变明显较轻,肾组织纤维化显著减轻(平均每肾羟脯氨酸含量73515μg±37717μgvs154013μg±35819μg),肾组织细胞NF-κBP65丢失情况明显减轻。结论:槲皮素对大鼠实验性慢性肾功能衰竭具有显著防治作用,治疗后肾脏病理改变显著减轻。

关键词:槲皮素;慢性肾功能衰竭;肾水肿;纤维化;NF-κB中图分类号:R25615;R69215　　文献标识码:A　　文章编号:1004-0668(2007)02-0048-03

EffectsofQuercetinontheRenalPathologicalChangesinRatswith

ExperimentalChronicRenalFailure

WangTian-ran,ZhouWei,ZengXiang-yuan,etal

(ExperimentalCentre,GeneralHospitalofChengduMilitaryArea,Chengdu,610083)

Abstract:objective:ThepreventionandcuringeffectsofQuercetinonrenalpathologicalchangesinratswithexperimentalchronicrenalfailurestudied1Methods:Theratsincontrolandtreatinggroupswerefedwith0175%adenineinfeedfor2monthtobebuildupchronicrenalfailure,andthequercetinwereadministeredorally(100mg/Kg d)for42daystotheratsintreatinggroup,beginningatthe15thday1Thebodyandkidneyweightsofthesurvivedratswerepreciselymeasuredandthekidney-indexeswerecalculatedattheendofthetreatingperiod1Therenal-moisturecapacitiesweredeterminedwithabakingmethod,andtherenalpathologicalinspectswereexecutedroutinely1Therenaltissuesclerosiswasassayedbydeterminingitshydroxyproline(Hyp)1TheNF-κBintherenalcellswereassayedwithaimmunochemicalassay1Results:Whencomparedwiththecontrolgroup,significantly(P<0101orP<01001),thekidney-indexesandtherenalmoisturecapacitiesweredecreased(5108%±0189&78124%±1125%vs12163%±2194&79129%±0190%),therenalpathologicalchangeswerelessserious,therenaltissuesclerosischangeswerereduced(meanquantityofHypperkidney,73515μg±37717μgvs154013μg±35819μg),andthelossesofNF-κBP65intherenalcellswerelessserious1Conclusion:Theresultssuggestedthatqercetinisofpreventionandcuringeffectsonchronicrenalfailureinrats,thepatholog2icalchangeswereevidentlyreducedafterquercetintreatment1

Keywords:quercetin;chronicrenalfailure;preventionandcuringeffect;renaloedema;sclerosis;NF-κB

作者简介:王天然,男,1957年生;主任技师;研究方向:药理学、临床免疫学、临床生物化学、肿瘤实验室诊断等。