pGEX_vector_map-表达载体

pGEX_vector_map-表达载体

GE Healthcare

pGEX vectors, GST Gene Fusion System

Map of the glutathione S-transferase fusion vectors showing reading frames and main features. Even though stop codons in all three frames are not depicted in this map, all thirteen vectors have stop codons in all three frames downstream from the multiple cloning site.

Ordering information

Product

Quantity

Code no.

pGEX-1λT EcoRI/BAP 5 µg 28-9546-56pGEX-2T 25 µg 28-9546-53pGEX-2TK 25 µg 28-9546-46pGEX-3X 25 µg 28-9546-54pGEX-4T-1 25 µg 28-9545-49pGEX-4T-2 25 µg 28-9545-50pGEX-4T-3 25 µg 28-9545-52pGEX-5X-1 25 µg 28-9545-53pGEX-5X-2 25 µg 28-9545-54pGEX-5X-3 25 µg 28-9545-55pGEX-6P-1 25 µg 28-9546-48pGEX-6P-2 25 µg 28-9546-50pGEX-6P-3

25 µg

28-9546-51

Do you want to learn more? Read the GST Gene Fusion System Handbook (18-1142-75). Please

contact your local GE Healthcare representative for a printed copy.

imagination at work

pGEX-1λT

Thrombin

↓CTG GTT CCG CGT GGA TCC CCG GAA TTC ATC GTG ACT GAC TGA CGA

BamHI

EcoRIStop codonspGEX-2T

Thrombin

↓CTG GTT CCG CGT GGA TCC CCG GGA ATT CAT CGT GAC TGA CTG ACG

Ile His Arg Asp

BamHISmaIEcoRI

Stop codonspGEX-2TK

ThrombinKinase

↓CTG GTT CCG CGT GGA TCT CGT CGT GCA TCT GTT GGA TCC CCG GGA ATT CAT CGT GAC TGA

BamHIpGEX-4T-1

SmaIEcoRI

Stop codonsThrombin

↓BamHI

EcoRISmaISalIXhoINotIStop codonspGEX-4T-2

Thrombin

↓CTG GTT CCG CGT GGA TCC CCA GGA ATT CCC GGG TCG ACT CGA GCG GCC GCA TCG TGA

Pro Gly Ile Pro Gly Ser Thr Arg Ala Ala Ala Ser

BamHIEcoRISmaISalIXhoI

NotIStop codonpGEX-4T-3

Thrombin

CTG GTT CCG CGT GGA TCC CCG AAT TCC CGG GTC GAC TCG AGC GGC CGC ATC GTG ACT GAC TGA

↓BamHIEcoRISmaISalIXhoI

NotIStop codonspGEX-3X

Factor Xa

ATC GAA GGT CGT Gly GGG ATC CCC GGG AAT TCA TCG TGA CTG ACT GAC

Ile Pro Gly Asn Ser Ser

BamHISmaIEcoRI

Stop codonspGEX-5X-1

Factor Xa

ATC GAA GGT CGT Ile Glu Gly Arg GGG ATC CCC GAA TTC CCG GGT CGA CTC Gly Ile Pro Glu Phe Pro Gly Arg Leu Glu GAG CGG CCG CAT CGT GAC TGA

Arg Pro His Arg Asp

BamHIEcoRISmaI

SalIXhoINotIStop codonspGEX-5X-2

Factor Xa

ATC GAA GGT CGT Ile Glu Gly Arg GGG ATC CCC GGA ATT CCC GGG TCG ACT CGA GCG GCC GCA TCG TGA

Gly Ile Pro Gly Ile Pro Gly Ser Thr Arg Ala Ala Ala Ser

BamHIEcoRISmaI

SalIXhoINotIStop codonpGEX-5X-3

Factor Xa

ATC GAA GGT CGT GGG ATC CCC AGG AAT TCC CGG GTC GAC TCG AGC GGC CGC ATC GTG ACT GAC TGA

Ile Pro Arg Asn Ser Arg Val Asp Ser Ser Gly Arg Ile Val Thr Asp

BamHIEcoRISmaISalIXhoINotI

Stop codonspGEX-6P-1

PreScission Protease

↓ Ser Pro Glu Phe Pro Gly Arg Leu Glu Arg Pro His

CTG GAA GTT CTG TTC CAG GGG CCC CTG GGA TCC CCG GAA TTC CCG GGT CGA CTC GAG CGG CCG CAT

BamHIEcoRI

SalI

pGEX-6P-2

SmaI

XhoI

NotI

PreScission Protease

Leu Glu Val Leu Phe Gln Gly ↓CTG GAA GTT CTG TTC CAG GGG CCC CTG GGA TCC CCA GGA ATT CCC GGG TCG ACT CGA GCG GCC GCA TCG

Pro Leu Gly Ser Pro Gly Ile Pro Gly Ser Thr Arg Ala Ala Ala Ser

BamHIEcoRI

SmaI

SalI

XhoI

NotI

pGEX-6P-3

PreScission Protease

Leu Glu Val Leu Phe Gln Gly Pro Leu Gly ↓CTG GAA GTT CTG TTC CAG GGG CCC Ser Pro Asn Ser Arg Val Asp Ser Ser Gly Arg

BamHIEcoRISmaISalIXhoI

NotITth111I

BalIaAatII

io

e S-transfersehnut

at

PtacgBspMI

ApSj10 Bam7Stop7

m

pr

PstI

pGEX

~4900 bp

NarIEcoRV

acI

q

p4.5

AlwNI

BssHII

ApaI

BstEII

pBR322MluI

ori