附录 I:常用荧光染料的激发及发射波长
Fluorescent Dye (荧光染料) Cy2 TM
GFP(Red Shifted) YO-PRO TM -1 YOYO TM -1 Calcein FITC FluorX TM Alexa TM 488 Rhodamine 110 ABI,5-FAM Oregon Green TM 500 Oregon Green TM 488 RlboGreen TM Rhodamine Green TM Rhodamine123 Magnesium Green TM Calcium Green TM TO-PRO TM -1
Excitation (激发波长)
489 488 491 491 494 494 494 490 496 494 503 496 500 502 507 506 506 514
Emission (发射波长)
506 507 509 509 517 518 519 520 520 522 522 524 525 527 529 531 533 533
Fluorescent Dye (荧光染料) TOTO?-1 ABI,JOE BODIPY?530/550 Dil BODIPY?TMR BODIPY?558/568 BODIPY?564/570 Cy3 TM Alexa TM 546 TRITC
Magnesium Orange TM Phycoerythrin,R & B Rhodamine Phalloidin Calcium Orange TM Pyronin Y Rhodamine B ABI,TAMRA Rhodamine Red TM
Excitation (激发波长)
514 520 530 549 542 558 564 550 555 547 550 565 550 549 555 555 560 570
Emission (发射波长)
533 548 550 565 568 568 570 570 570 572 575 575 575 576 580 580 582 590
Fluorescent Dye (荧光染料) Cy3.5 TM ABI,ROX
Calcium Crimson TM Alexa TM 594 Texas Red? Nile Red YO-PRO TM -3 YOYO TM -3 R-Phycocyanin C-Phycocyanin TO-PRO TM -3 TOTO?-3 DiD DilC’(5) Cy5 TM
Thiadicarbocyanine Cy5.5
Excitation Fluorescent
(激发波长)Dye (荧光染料)
581 588 590 590 595 549 612 612 618 620 642 642 644 649 651 675
596 608 615 615 615 628 631 631 642 648 660 660 665 670 671 694