拟南芥种植手册_tair(11)

原版拟南芥规范种植手册

Equipment:

Heat resistant dishes with cover, analytical balance, forced draft oven,s desiccator with silica gel, tongs and oven cloth.

Method:

1. Weigh one clean numbered dish and cover accurately to 4 decimal places using an analytical balance. Write the weight (W1) in the notebook.

2. Add approximately 100 or 200 mg of seeds distributed evenly over the base of the dish, replace the cover and accurately weigh the dish and cover. Write this weight (W2) in the notebook.

3. Place the dish in a safe place and continue to do the second and/or third replicates in the same way.

4. When all samples have been weighed into numbered dishes, place each dish on top of its numbered lid in the oven at 100-105°C.

5. Wait for the oven to reach this temperature and heat the samples for 15-17 hours.

6. Remove the dishes from the oven, replace their covers and place in a desiccator to cool for 30 to 45 minutes at room temperature. After heating, make sure that the dishes are put directly into the desiccator so that the dry seeds do not absorb more moisture.

7. Remove the dishes one by one from the desiccator and immediately weigh each dish and cover, and write the weight (W3) in the notebook. Do not leave the desiccator open during the weighings.

8. Moisture content is calculated as the loss in weight as a percentage of the original

weight of seeds. This is known as wet basis or fresh-weight basis, and is expressed to one decimal place. Algebraically, if W1 is the weight of the dish, W2 the weight of dish and seed before drying, and W3 the weight of dish and seed after drying, then:

% Moisture Content = 100 x ( W2 - W3 ) / ( W2 - W1 )

After seed moisture content is within the safe storage limits, dried seeds should be placed in tightly sealed and impermeable containers to prevent rehydration. Cryovials (with threaded lids and gaskets) are convenient for storage. They hold large numbers of seeds, seal tightly and can be resealed many times.

In packaging seeds, each container should be labeled with relevant information including date of storage using a waterproof permanent marker, or a suitable printed label. In determining seed quantities, approx 1250 seeds = 25 mg = 50 microliters. Seal the