拟南芥种植手册_tair(13)

原版拟南芥规范种植手册

recognized. A germination test is the best method of estimating seed viability.

Arabidopsis seeds may fail to germinate because they are dormant or because they are dead. Dormant seeds typically remain firm and in good condition during the germination test while dead seeds soften and are attacked by fungi. Imbibing seeds with water at low (refrigerator) temperatures can usually break dormancy.

The following method to test seed viability is suitable for Arabidopsis:

1. Place two layers of filter paper (free from chemical residues that could interfere with the germination of the seeds) firmly in the bottom of a 10-cm diameter petri dish, labeled with line number and date.

2. Moisten the paper with distilled water. The paper should be totally saturated, but no excess water should be left in the dish.

3. Distribute 100 seeds uniformly on the surface of the paper. Replace the lid and seal the dish with Parafilm or clear tape, to preclude desiccation.

4. Cold treat seeds by placing petri dishes in the refrigerator for 2-4 days.

5. Place the dishes on an illuminated shelf (or in a growth chamber) under standard light and temperature conditions for Arabidopsis.

6. After 3 to 6 days, count germinated vs. un-germinated seeds, and record germination percentage.

These methods are used by the ABRC for handling plants and seeds. If you have any questions concerning the above procedures, feel free to contact us at .

Arabidopsis Biological Resource Center

Ohio State University

1060 Carmack Road

Columbus OH 43210

Phone: 614-292-9371

Fax: 614-292-0603

Email: abrc@