拟南芥种植手册_tair(8)

原版拟南芥规范种植手册

Commercial seed collectors: AraconsTM (Lehle Seed Co. Catalog) placed soon after bolting are effective for single plant harvesting, preventing cross pollination and seed contamination, but are not necessary when simple bulk production is desired. Harvesting after pots have been allowed to dry is accomplished easily by carefully cutting off the inflorescence under the cone device, placing the

AraconsTM plus contents carefully on a large plastic bag (approx 4 liter) or a large piece of paper, removing the plant material from the plastic cylinder, and then shaking the seeds into the bag or paper. Alternatively, if plants are totally dry, the plant material can be placed directly onto threshing sieve (see threshing section below).

Use of plastic floral sleeves: For bulk seed production using individual pots, the pots can be placed into transparent clear plastic (i.e. polypropylene) floral sleeves near the bolting time. These sleeves fit snugly around a 10-cm pot, extend 50-60 cm upward, and are wider at the top allowing for expansion of the developing inflorescences, maintain upright stiffness, and tear easily for harvesting. All plant inflorescences are maintained within the sleeve, forming a propagator for each pot. At harvest, the sleeves can be cut or torn, the inflorescences cut off at the base, and the plant material placed into plastic bags or, if plants are totally dry directly onto threshing sieve. This method is very effective for achieving high densities of lines while maintaining productivity and purity of each pot.

Bagging inflorescences by pot: Inflorescences can simply be trained into an

approx 4 liter transparent plastic bag before any siliques begin to brown. The bag, however, may potentially collect moisture from transpiration or careless watering and provides a haven for insects when greenhouses are sprayed. To reduce these possibilities, the tops of these bags should be kept widely open at all times. Wait until the inflorescence has browned before harvesting. This method is conducive to strict isolation of the lines, and the bag serves to collect shattered seeds.

Harvesting is accomplished by carefully cutting the entire inflorescence off at its base after all seeds have matured and shaking the seeds into the plastic bag.

Bulk production on the open bench: For bulk seed production, the best method is to simply grow the plants on the open bench, keep all lines separated by adequate space, avoid disturbance of maturing inflorescences, and harvest when all siliques are dry. The entire inflorescence is cut off at its base and carefully placed into an approx 4 liter or larger transparent plastic bag, depending on the size of the bulk of plants. This is compatible with the goals of high seed quality, maximum seed yield, and good pest protection. Some seeds may be lost, but the remainder are almost always healthy, and result in vigorously germinating seedlings. After

harvest, the entire contents of the bag are allowed to dry in preparation for

threshing.

Early harvest of individual siliques: Seeds from individual siliques can be

harvested after the siliques have turned completely yellow, if rapid turnover is required. However, such seeds do have high levels of germination inhibitors. For