拟南芥种植手册_tair(5)

原版拟南芥规范种植手册

Water requirement is strongly influenced by relative humidity. Arabidopsis

plants, including seedlings, tolerate low humidity (e.g., 20-30%) although

increased humidity (e.g., 50-60%) greatly reduces the risk of accidental drying of the soil surface and subsequent desiccation of the fragile, germinating seedlings. Very high humidity (more than 90%) can induce the formation of mold. Low

humidity (less than 50%) is desirable when siliques begin to mature.

Poor nutrition can lead to rapid flowering, short growth period and low seed set. If an extended time release fertilizer was not utilized before, a mild mineral nutrient solution can be applied to the pots at 2-week intervals (5 mM KNO3, 2.5 mM KH2PO4 (adjusted to pH 6.5), 2.0 mM MgSO4, 2.0 mM Ca (NO3)2, 50 microM Fe-EDTA, 70 microM H3BO3, 14 microM MnCl2, 0.5 microM CuSO4, 1

microM ZnSO4, 0.2 microM Na2MoO4, 10 microM NaCl, 0.01 microM CoCl2, pH 6.5).

Light

Optimum light is approx 130-150 uE m-2 sec-1. Very high output or cool white (VHO or SHO) fluorescent lamps, supplemented by incandescent lighting are used for growth chambers. Older plants tolerate higher light intensity, up to full sun, although the use of 60% shade cloth in summer greenhouses helps with light intensity control and temperature regulation. Supplemental evening and morning light is provided in the greenhouse during winter since the plants generally require a long photoperiod (at least 12 hours) for flowering. Photoperiods of 16 hours work well for greenhouse growth. Plants flower rapidly under continuous light or long days, while under short days flowering is prevented or delayed, favoring growth of vegetative tissue. Continuous light is well-tolerated and can be used to accelerate the reproductive cycle.

Temperature

The optimum growth temperature range for Arabidopsis is 23-25°C. In general, high temperatures favor a reduced number of leaves and flowers, and fertility is reduced. At lower temperatures, growth is slow and flowering is delayed. Lower temperatures are permissible, but higher temperatures are not recommended,

especially for germination through early rosette development. Older plants

tolerate higher temperatures, at least up to 30°C. It is advisable to set the

greenhouse temperature at 21-23°C to avoid fluctuations to higher temperatures. It is recommended that night temperatures be maintained 2-4°C lower than the day temperature. Some late flowering natural accessions (ecotypes) require an additional 4°C incubation (vernalization) of young rosettes for 3-4 weeks to

induce flowering.

Control of pests