重组人骨形成蛋白2骨诱导中神经生长因子及其受(4)

目的 观察重组人骨形成蛋白2(recombined human bone morphogenetic protein 2, rhBMP-2)异位诱导成骨过程中神经生长因子(nerve growth factor, NGF)及其高亲和力受体(tyrosine kinase receptor A, TrkA)的表达,探讨NGF在BMP骨诱导中的作用。 方法 ICR小鼠36只,随机分

图1　实验组术后组织学观察(HE ×100)　○a 　7d 大量软骨细胞形成　○b 　14d 大量小梁状骨结构形成,可见原始骨髓腔、骨髓细胞　○

c 　21

d 大量板层骨形成,伴有成熟骨髓组织　图2　对照组术后21d ,未见新骨形成(HE ×100)　图3　实验组NGF 及TrkA 免疫组织化学染色　○

a 　7d 成软骨细胞、软骨细胞、肥大软骨细胞和成骨细胞中均有广泛的N GF 阳性染色(×200)　○

b 　14d 在部分成骨细胞、成骨样细胞和幼稚骨细胞中有N GF

阳性染色(×200)　○c 　21d 仅在少数成骨样细胞和破骨细胞中有N GF 阳性染色(×320)　○d 　7d 成软骨细胞、软骨细胞、肥大软骨细胞和成骨细

胞中均有T rkA 阳性染色(×200)　○e 　14d 在部分成骨细胞、成骨样细胞和幼稚骨细胞中有T rkA 阳性染色(×200)　○

f 　21d 仅在少数成骨样细胞和破骨细胞中有T rkA 阳性染色(×320)　图4　rhB M P -2诱导新骨中NGF 及G 3PD H mRNA 的表达　M :M arker

F ig .1　H istolog ical observation af ter i m plan tation i n the exper i m en tal group (HE ×100)　○

a 　N um erous chondrocytes w ere fo rm ed in the experi m ental group on the 7th day 　○

b 　N um erous trabecular bones w ere fo rm ed w ith p ri m ary m arrow cavities in the experi m ental group on the 14th

day 　○b 　N um erous lam ellar bones w ith m ature bone m arrow w ere fo rm ed in the experi m ental group on the 21st day 　F ig .2　No new bone for mation was found i n the con trol group on the 21st day (HE ×100)　F ig .3　I mmunoh istoche m ical localization of NGF and TrkA i n the

exper i m en tal group 　○

a 　Chondroblasts ,chondrocytes ,hypertroph ic chondrocytes ,and o steoblasts w ere stained fo r N GF on the 7th day (×200)　○

b 　Po sitive staining of N GF w as found in part of the o steoblasts ,o steoblast 2like cells ,young o steocytes ,and o steoclasts on the 14th day (×200)○

c 　A few o steoblast 2like cells an

d o steoclasts exp ressed po sitiv

e staining fo r N GF on the 21st day (×320)　○d 　Chondroblasts ,chondrocytes ,hypertroph ic chondrocytes,and o steoblasts w ere stained fo r T rkA on the 7th day (×200)　○

e 　Po sitive staining o

f T rkA w as found in part of the o steoblasts,o steoblast 2like cells,youn

g o steocytes,and o steoclasts on the 14t

h day (×200)　○

f 　A few o steoblast 2like cells and o steoclasts exp ressed po sitive stainin

g fo r T rkA on the 21st day (×320)　F ig .4　Expression of mRNA of NGF and G3PD H i n new bone i nduced by rhB M P -2M :M arker ・

754・中国修复重建外科杂志2006年第20卷第4期