水分胁迫对番茄幼苗叶片和根系中多胺代谢的影

第37卷　第7期2009年7月西北农林科技大学学报(自然科学版)

JournalofNorthwestA&FUniversity(Nat.Sci.Ed.)Vol.37No.7

Jul.2009

水分胁迫对番茄幼苗叶片和根系

中多胺代谢的影响

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张志新1,邹志荣1,张春梅1,2,王云冰1,杨建军1,燕　飞1

(1西北农林科技大学园艺学院,陕西杨凌712100;2河西学院农学系,甘肃张掖734000)

[摘　要]　【目的】探讨番茄体内多胺代谢与抗旱性的关系,为植物抗旱性的深入研究提供理论依据。【方法】

以番茄幼苗为材料,用不同质量浓度(0(CK),50,75,100g/L)的聚乙二醇(PEG6000)模拟水分胁迫,研究不同水分胁迫条件下番茄幼苗叶片和根系中多胺代谢的变化。【结果】水分胁迫影响番茄幼苗叶片和根系中的多胺代谢,随胁迫时间的延长,番茄幼苗叶片和根系中游离态腐胺(Put)、亚精胺(Spd)、精胺(Spm)及精氨酸脱羧酶(ADC)、鸟氨酸脱羧酶(ODC)、多胺氧化酶(PAO)活性均呈先上升后下降的趋势,且除PAO外上升幅度随胁迫程度加重而增大。番茄幼苗叶片和根系中ADC、ODC活性与Put含量极显著正相关,水分胁迫下ADC、ODC的合成代谢;

PAO活性与Spd、Spm含量变化趋势相似且极显著相关,茄幼苗中多胺代谢活跃,,。

[关键词]　番茄;水分胁迫;多胺代谢;酶活性;[中图分类号]　S641.201

[[]　167129387(2009)0720097206

Eonpolyaminesmetabolisminleavesand

rootsoftomatoseedlings

ZHANGZhi2xin1,ZOUZhi2rong1,ZHANGChun2mei1,2,

WANGYun2bing1,YANGJian2jun1,YANFei1

(1CollegeofHorticulture,NorthwestA&FUniversity,Yangling,Shaanxi712100,China;

2DepartmentofAgriculturalScience,HexiUniversity,Zhangye,Gansu734000,China)

Abstract:【Objective】Thestudywasdonetoevaluatetherelationshipbetweenpolyaminesmetabolismandtheresistancetodroughtintomato,andtoprovidetheoreticalfoundationforfurtherresearchofplants’drought2resistance.【Method】Thechangesincontentsofendogenouspolyamine(PAs)andmeta2bolicenzymeactivitiesinleavesandrootsoftomatoseedlingsgrownbyhydroponiccultivationunderdiffer2entlevelsofwaterstress,whichwereinducedbydifferentpolyethyleneglycol(PEG6000)concentrationsas0(CK),50,75and100g/Lrespectively,werestudied.【Result】Polyaminesmetabolisminleavesandrootsoftomatoseedlingswasaffectedbywaterstress.ThecontentsofPAs(Put,Spd,Spm)andactivitiesofADC,ODCandPAOinleavesandrootsoftomatoseedlingsshowedatrendofrisefirst,thenfellwiththeprolongationoftime.Withthewaterstressdegreeintensifying,therisingamplitudeofPAscontentsandactivitiesofADCandODCincreased.【Conclusion】TherewasasignificantpositivecorrelationbetweenPutcontentsandtheactivitiesofADCandODCintheleavesandroots,whichsuggestedthatbothADCandODCarethemajorpathwayofPutbiosynthesisintomatoseedlingsunderwaterstress.Moreover,it

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[基金项目][作者简介][通信作者]

　2008211203　国家“十一五”科技支撑项目(2007BAD79B04)　张志新(1982-),男,山西五台人,在读硕士,主要从事设施园艺研究。E2mail:sxtuzzx@　邹志荣(1956-),男,陕西延安人,教授,博士生导师,主要从事设施园艺研究。E2mail:zouzhirong2005@