脂肪干细胞构建组织工程化角膜基质组织(4)

634复旦学报(医学版) 2010年11月,37(6)

料残存于新生角膜纤维间,24周时可见缺损周围胶原纤维排列方向改变,无规则板层结构。随机抽取每组角膜纤维各300次,测得rASCs PLGA组胶原纤维直径平均为(31.4(4.0)nm,PLGA组胶原纤维直径平均为(33.3(5.5)nm,正常兔角膜胶原纤维直径为

(30.9(3.3)nm,t检验进行统计学分析,结果提示rASCs PLGA组胶原纤维直径与正常兔角膜胶原纤维直径分布差异无统计学意义(P=0.08),PLGA组胶原纤维直径与正常角膜胶原纤维直径差异有统计学意义(P<0.05)

。

图2 术后0、12、24周兔角膜大体观

Fig2 Grossviewofrabbitcorneaat0,12and24weekspost implantation

0week:Whitishopaquediskofgraftswithinthecornea;12weeks:PLGAmaterialwasmostlydegradedandthecorneabecamenearlytransparentinrASCs+PLGAgroupwhilepartialmaterialretainedinPLGAalonegroup;24weeks:Thecorneaofbothgroupsbecametransparen

t.

图3 移植12和24周后组织形态学观察(黑色箭头示手术区域)

Fig3 Histologicalevaluationoftheengineeredcornealstromaat12and24weeksaftertransplantation

A1,A2:EngineeredcorneainrASCs+PLGAgroupat12(A1)and24weeks(A2)aftertransplantation;B1,B2:EngineeredcorneainPLGAgroupat12(B1)and24weeks(B2)aftertransplantation.Arrowsshowoperation

area.

图4 移植12和24周后的透射电镜观和胶原纤维直径检测(&12000)

Fig4 TEMevaluationofdiameterofcollagenfibrilsineachgroupat12weeks(A1,B1)

and24weeks(A2,B2)afterimplantation(&12000)

A1:Fewerincreasedinter fibrilspacinginrASCs PLGAgroupat12weeksaftertransplantation;A2:NearlynormalcollagenfibrilsdistributioninrASCs PLGAgroupat24weeksaftertransplantation;B1:PLGAmaterialamongcollagefibrilsinPLGAgroupat12weeksaftertransplantation;B2:InterlacedcollagenfibrilsinPLGAgroupat24weeksaftertransplantation;of