Growth and accelerated differentiation of mesenchymal stem c(5)

PaperJournalofMaterialsChemistryB

25/05/2015 04:20:30.

Fig.6Immuno uorescent(A)CD-44and(B)OCNstainingofMSCsgrownonthe(GO/PLL)4 lm(left)andontheTCPS(right).Thehistogramistheratioofpositivelystainedcellsandthesumofposi-tivelyandnegativelystainedcellsonallthestudiedsubstratesbyday2,7,and12,

respectively.

theGO/PLLlmbyday7,ascomparedtothoseonTCPS,showingnearly55%positiveOCNwhilethelattershowed35%positive.Takentogether,theseresultsindicatedthattheGO/PLLlmcouldpromotetheosteogenesisofMSCssignicantly.Herein,thepredominanceoftheGO/PLLcompositelmsovertheGOsurfaceandthePLLsurfacemightbetheresultsofcoupledcombinationoftwocomponents,whichcouldbeevi-dencedbythefollowingstudy.

Sincetheconventionalosteogenicmediacandirectlymediatethedi erentiation,adirectcorrelationwiththeadsorptionofthesubstratefortheosteogenicchemicalinducerswasthoughttobeinvolved.15,31Andwhatismore,previousstudieshaverecognizedthatGOhastheremarkableloadingcapacitiesforproteins,DNAandorso,viaitslarge

Thisjournalis©TheRoyalSocietyofChemistry2014Fig.7Loadingcapacityof(A)dexamethasone,(B)b-glycerolphos-phate,(C)ascorbicacidindi erentconcentrationonallthestudiedsurfaces.Theinsetsarethechemicalstructures,respectively.

surfaceareaandintermolecularinteraction.32–34Therefore,theadsorptionofthe(GO/PLL)4lmforthechemicalinducerswasinvestigatedusingUVspectrophotometry.First,theloadingcapacitiesofthelmfortheosteogenicchemicals,dexameth-asone,b-glycerolphosphate,ascorbicacid,weredeterminedwiththeadsorptionkineticsrecordedinFig.S8. Furthermore,theadsorptionofthechemicalsonthestudiedsubstrateswasalsodeterminedwithincreasingconcentrationof

the

J.Mater.Chem.B,2014,2,5461–5467|5465

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