基于HLA组特异性单倍体全长测序方法对模棱两可(8)

背景：传统的HLA测序分型技术都是对父系母系双倍体进行扩增,由于HLA多样性高,产生了大量的模棱两可难题,而采用基于组特异性单倍体全长测序分型方法来解决模棱两可结果的确认定型鲜有报道。目的：分析基于组特异性单倍体全长测序分型方法对HLA基因分型模棱两可结果准确定型的效果。方法：对2例模棱两可结果标本以低分辨率分型方法结果作为参考起点,通过组特异性扩增分离,基于等位基因全长单倍体的Sanger测序(PCR-SBT)分型。

王宋兴，等. 基于HLA 组特异性单倍体全长测序方法对模棱两可结果的准确定型 ISSN 2095-4344 CN 21-1581/R CODEN: ZLKHAH

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