201012-1335嗯嗯呃

嗯嗯呃

- 1 - 中国科技论文在线Identification of trans-tiliroside as active principle with anti-hyperglycemic,anti-hyperlipedemic and antioxidant

effects from Potentilla chinesis

Qiao Wei, Zhao Chuan, Qin Nan, Zhai Huiyuan, Duan Hongquan

(School of Pharmaceutical Sciences, Research center of Basic Medical Sciences,Tianjin Medical

University, TianJin 300070)

基金项目：Financially supported by specialized research Fund for the Doctoral Program of Higher Education （No.200800620007）

作者简介：乔卫，（1968-），女，副教授，主要研究方向：天然药物化学与中药药理学

通信联系人：段宏泉，（1965-），男，教授，主要研究方向：中药和天然药物活性物质的现代研究. E-mail: duanhq@

Abstract: Aim of the study: The present study was carried out to isolate and identify trans-tiliroside as principal compound with anti-hyperglycemic, anti-hyperlipidemic and antioxidant effects from Potentilla chinesis. Methods: A bioactive compound, trans-tiliroside has been isolated from the ethanol extract of Potentilla chinesis.The normal, alloxan-induced diabetic mice and streptozotocin-induced diabetic rats were used to evaluate the anti-hyperglycemic, anti-hyperlipidemic and antioxidant effects of trans-tiliroside from Potentilla chinesis. Results: Trans-tiliroside in normal and diabetic mice significantly decrease the level of serum glucose levels, TG and TC in alloxan-induced diabetic mice. In streptozotocin induced diabetic rats, trans-tiliroside showed a significant decrease in blood glucose level. The content of TC, LDL-C and TG levels were decreased and high density lipoprotein HDL-C content was increased, so lipid metabolism was improved. Moreover, trans-tiliroside revealed can increased antioxidant activity in diabetic rats. Histological morphology examination showed that the trans-tiliroside restored the damage of pancreas tissues in rats with diabetes mellitus. Conclusion: Trans-tiliroside revealed significant anti-hyperglycemic, anti-hyperlipidemic and antioxidant activities. Keywords:trans-tiliroside; Potentilla chinesis; anti-hyperglycemic; anti-hyperlipidemic; antioxidant effects; active principle

0 Introduction

Diabetes mellitus (DM) is one of the most severe, incurable metabolic disorders characterized by hyperglycemia as a result of a relative, or an absolute, lack of insulin, or the action of insulin on its target tissue or both [1]. Besides hyperglycemia, several other symptoms, including hyperlipidemia, are involved in the development of microvascular complication of diabetes, which are the major causes of morbidity and death [2]. Because the mechanism of DM is quite complex, many currently available synthetic chemical antidiabetic agents have low rates of response and remission and even severe adverse-effects. Accordingly, it is necessary to research and develop more effective hypoglycemic agents with lower adverse-effect [3].

Potentilla chinesis Ser (Rosaceae) is a plant used in China and parts of Asia as a natural treatment for diarrhea, rheumatoid ache, paralysis, epilepsy, scabies, etc [4]. As a Chinese traditional medicine, it is chewed as hypoglycemic herb by native people of China [5] In the previous phytochemical investigations of Potentilla chinesis, the isolation and structure elucidation of triterpenes, flavones and organic acid from the entire plant were reported [6,7]. More recently, our pharmacological screening studies indicated that the 95% ethanol-extracts and EtOAc fraction of Potentilla chinesis revealed significant hypoglycemic effects in alloxan-induced mice [8]. To the best of our knowledge, the principal compound with anti- hyperglycemic activity and its probable mode of action have not been studied so far. This paper deals with the anti-hyperglycemic, ant-hyperlipidemic and antioxidant effects of kaempferol -3-O-β-d-(6″-E-p-coumaroyl) glucopyranoside (trans-tiliroside) isolated from Potentilla chinesis.