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- 5 - 中国科技论文在线2.3 Anti-hyperglycemic effect in normal and STZ-induced diabetic rats

The anti-hyperglycemic effect of trans-tiliroside was further evaluated in STZ-induced diabetic rats. Blood glucose level was measured in normal and experimental rats on 0, 4, 8,12th weeks of treatment with trans-tiliroside. From Fig 2, it appears that administration of streptozotocin (60 mg/kg) led to over 5.5-fold elevation of blood glucose level in time-dependent manner (p <0.001), which was maintained over a period of 12 weeks. Treatment of STZ-induced diabetic rats with trans-tiliroside in the dose of 1.2 and 0.3 mg/kg, oral for 12 weeks resulted in a significant (p <0.01, 0.05) decrease in blood glucose level as compared to that of the diabetic control group. Treated control rats did not exhibit any significant alteration in their glucose levels through the duration of the experiment.

Fig.2. Effect of trans-tiliroside isolated from Potentilla chinesis on the blood glucose levels of the streptozotocin-induced diabetic rats after 10 weeks treatment. Values are presented as mean ±SEM, with 8 animals

for each group. * p <0.05, * p < 0.01 ,compared to diabetic control group

2.4 Effect of trans-tiliroside on serum lipids in normal and diabetic animals Table 3 shows the levels of serum TG and TC in normal and alloxan-induced diabetic mice in each group. The TG and TC levels of untreated diabetic control mice were significantly increased as compared to normal mice. When diabetic mice were treated for 15 days, the trans-tiliroside (1.6, 0.8 and 0.4 mg/kg) significantly (p <0.05) dose-related decreased the levels of TG and TC. After 15 days treatment of the tested compound, the serum lipids of diabetic mice restored to near normal level. Alloxan-induced diabetic mice treated with trans-tiliroside were comparable to those in diabetic rats treated with metformin. Normal rats treated with trans-tiliroside alone showed no significant difference in lipid levels as compared to normal rats.

Table 4 shows the levels of serum lipids (TC, TG, LDL-C, HDL-C) in normal and STZ-induced diabetic rats in each group. TC, TG, LDL-C levels were significantly increased while HDL-C level was decreased in STZ-induced diabetic rats as compared to normal rats. Treatment of diabetic rats with trans-tiliroside for 12 weeks resulted in marked dose-dependent decrease in serum TC, TG, LDL-C levels and dose-dependent increase in HDL-C levels as compared to STZ-induced diabetic rats. The lipid profiles in each trans-tiliroside treated group was similar to those in the metformin control group (p >0.05 for TC, TG and LDL-C, respectively). Oral administration of trans-tiliroside for 12 weeks significantly restored the levels of serum lipids to near normal in diabetic rats (Table 4).