201012-1335嗯嗯呃(9)

嗯嗯呃

- 9 - 中国科技论文在线and superoxide anion scavenging activities) of trans-tiliroside have previously been reported [19,34]. In this paper, we further explored the antioxidant effect in diabetic rats. The treatment with trans-tiliroside (1.2 and 0.3 mg/kg) for 12 weeks could efficiently restored the depleted status of the antioxidant enzymes SOD and lowered MDA levels on STZ-diabetic rats. The result illustrated that the compound can protect against STZ-diabetic oxidant tissue damage and this could be due to the presence of flavnoid structure. Our study together with existing reports thus suggests that trans-tiliroside possesses in vivo antioxidant property in diabetic condition.

4 Conclusions

In summary, trans-tiliroside as a principal compound from Potentilla chinesis exert a benefcial effects on hyperlipidemia due to diabetes and show a significant anti-hyperglycemic, anti-hyperlipidemic, antioxidant activity in diabetic animals. Histological examination also showed the trans-tiliroside evidently reduced the injuries of pancreas induced by streptozotocin. This has clinical implications, that the trans-tiliroside, if used as an anti-hyperglycemic agent, may also reverse dyslipidemia associated with diabetes, and prevent the coronary risks which are very prevalent in diabetic patients. Further pharmacological investigation are in progress to expect this compound can be used as a drug.

5 Acknowledgements

This work was supported by the Foundation for the National Natural Science Foundation of China (No.NSFC30772635) and supported by science foundation of ministry of education of china.

References

[1] Ahmad, F., Azevedo, J.L., Cortright, R., et al. Alterationsin in skeletal muscle protein- tyrosine phosphatase activity and expression in insulin-resistant human obesity and diabetes[J].Journal of Clinical Investigation 1997,100: 449～458.

[2] Taskinen, M.R.. Diabetic dyslipidemia[J]. Atherosclerosis Supplements, 2002, 3: 47～51.

[3] Sun, J.E., Ao, Z.H., Lu, Z.M., et al. Antihyperglycemic and antilipidperoxidative effects of dry matter of culture broth of Inonotus obliquus in submerged culture on normal and alloxan-diabetes mice[J]. Journal of Ethnopharmacology, 2008, 118: 7～13.

[4] Tomczyk M., Latte K.P.Potentilla —A review of its phytochemical and pharmacological profile[J]. Journal of Ethnopharmacology, 2009,122: 184～204.

[5] Ma Y., Wen S.Z..

Efficacy of in patients with type 2 diabete s mellitus[J]. Chinese Traditional and Herbal Drugs, 2002,33,644.

[6] Liu P., Duan H.Q.,Pan Q.,Zhang Y.W., Yao Z.,. Triterpenes from herb of Potentilla chinesis[J]. Zhongguo Zhong Yao Za Zhi, 2006, 31: 1875～1879.

[7] Wang Q.H., Li Z.Y.，Shen Y., et al. Studies on triterpenoids from Potentilla chinensis[J]. Zhongguo Zhong Yao Za Zhi, 2006, 31: 1434～1436.

[8] Zhao C., Qiao W., Zhang Y., et al. Study on anti-diabetes active fraction and constituents from Potentilla chinesis[J]. Zhongguo Zhong Yao Za Zhi, 2008, 33: 680～682.

[9] Tsukamoto S., Tomise K., Aburatani M. Isolation of Cytochrome P450 Inhibitors from Strawberry Fruit, Fragaria ananassa[J]. Journal of Natural Products, 2004, 67: 1839～1841.

[10] Ravi,K., Ramachandran,B., Subramanian,S. Protective effect of Eugenia jambolana seed kernel on tissue antioxidants in streptozotocin-induced diabetic rats. Biological & Pharmaceutical Bulletin[J].2004,27:1212～1217.

[11] Li Q.G., Sun R., Gao F.Z.. Effect of Shen Di Jiang Tang Granules on diabetic rats[J]. Zhongguo Zhong Yao Za Zhi, 2001,26: 488～490.

[12] Nijveldt RJ, van Nood E, van Hoorn DEC, Boelens PG, van Norren K, van Leeuwen PAM.Flavonoid: a review of probable mechanisms of action and potent applications[J].American Journal of Clinical Nutrition, 2001, 74: 418～425.

[13] Tringali C. Bioactive compounds from natural sources (isolation, characterization and biological properties)[M]. London: Taylor & Francis group; 2001.