siRNA非病毒递送载体的研究现状(6)

siRNA 非病毒 递送载体 现状

杨飞飞等: siRNA非病毒递送载体的研究现状

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靶基因的沉默效果, 又避免siRNA诱导的免疫刺激和脱靶副作用, 为其临床应用打下基础[65]。

最近, 牛津大学科学家开发出一种由DNA制造的分子“笼子”, 能进入哺乳动物活细胞内部并在其中生存, 可能带来一种有效的药物递送新方法。DNA“分子笼”由4条人工合成的DNA短链构成, 能围绕蛋白质分子组装起来, 从而形成一个“分子笼”将该蛋白质包在内部, 而且能够通过设计程序, 让“分子笼”在遇到细胞内特定的“触发”分子时再度打开。特波菲尔德认为, 这种四面体“分子笼”只有7 nm, 不但容易进入细胞, 还留出了相当大的空间以容纳药物, 可以根据所装载的药物设计“分子笼”的结构, 以达到理想的释放

[66]

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efficient lipid-mediated DNA-transfection procedure [J]. Proc Natl Acad Sci USA, 1987, 84: 7413 7417.

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mediated RNA transfection [J]. Proc Natl Acad Sci USA, 1989, 86: 6077 6081.

[14] Shen Y, TU JS, Pang H, et al. Electrophoresis and fluorospec-trophotometry methods to determine the content and entrapment efficiency of siRNA in cationic liposomes [J]. Acta Pharm Sin (药学学报), 2009, 44: 430 435.

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persistent in vivo anti-HBV activity of chemically modified

。该研究为

siRNA的递送提供了新的思路和方法, “分子笼”在递送siRNA方面的应用有待研究开发。

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