抗苯磺隆猪殃殃乙酰乳酸合成酶的突变研究(5)

抗苯磺隆猪殃殃乙酰乳酸合成酶的突变研究

976 中 国 农 业 科 学 43卷

酰脲类除草剂产生抗药性的分子基础[24-25]。因此，该突变可能是猪殃殃对苯磺隆产生抗药性的重要原因。而发生在第457位和第573位的氨基酸突变是否也会导致猪殃殃对苯磺隆产生抗药性还需进一步研究。本试验尚未扩增出ALS基因的全长序列，特别是报道最多的第197位氨基酸是否发生变异还不清楚，因此有必要获取ALS基因全长，以便全面了解猪殃殃对苯磺隆的抗性机制。

4 结论

通过PCR技术，克隆出麦田恶性杂草猪殃殃ALS基因的部分片段，该片段共有3处位点发生了突变，其中Trp574的突变有可能是导致猪殃殃产生抗药性的重要原因之一。

References

[1] 彭学岗, 王金信, 段 敏, 杨纪辉. 中国北方部分冬麦区猪殃殃对

苯磺隆的抗性水平. 植物保护学报, 2008, 35(5): 459-462.

Peng X G, Wang J X, Duan M, Yang J H. The resistance to tribenuron-methyl in Galium aparine in winter wheat fields in northern China. Acta Phytophylacica Sinica, 2008, 35(5): 459-462. (in Chinese)

[2] Duggleby R G, Pang S S. Acetohydroxyacid synthase. Biochemistry

and Molecular Biology, 2000, 33: 1-36.

[3] Tan S Y, Evans R R, Dahmer M L, Singh B K, Shaner D L.

Imidazolinone-tolerant crops: History, current status and future. Pest Management Science, 2005, 61: 246-257.

[4] Sibony M, Michel A, Haas H U, Rubin B, Hukle K.

Sulfometuron-resistant Amaranthus retroflexus: Cross-resistance and molecular basis for resistance to acetolactate synthase-inhibiting herbicides. Weed Research, 2001, 41: 509-522.

[5] Durán-Prado M, Osuna M D, de Prado R, Franco A R. Molecular

basis of resistance to sulfonylureas in Papaver rhoeas. Pesticide Biochemistry and Physiology, 2004, 79(1): 10-17.

[6] Tranel P J, Wright T R. Resistance of weeds to ALS-inhibiting

herbicides: What have we learned? Weed Science, 2002, 50: 700-712. [7] Christopher J T, Powles S B, Holtum J A M. Resistance to

acetolactate synthase-inhibiting herbicides in annual ryegrass (Lolium rigidum) involves at least two mechanisms. Plant Physiology, 1992, 100: 1909-1913.

[8] Guttieri M J, Eberlein C V, Thill D C. Diverse mutations in the

acetolactate synthase gene confer chlorsulfuron resistance in kochia (Kochia scoparia) biotypes. Weed Science, 1995, 43 (2): 175-178.

[9] Tranel P J, Wright T R, Heap I M. ALS mutations from herbicide-

resistant weeds. URL http: //www. weedscience. org. Accessed: January 2008.

[10] YU Q, Zhang X Q, Hashem A, Walsh M J, Powles S B. ALS gene

proline (197) mutations confer ALS herbicide resistance in eight separated wild radish (Raphanus raphanistrum) populations. Weed Science, 2003, 51: 831-838.

[11] YU Q, Han H, Powles S B. Mutations of the ALS gene endowing

resistance to ALS-inhibiting herbicides in Lolium rigidum populations. Pest Management Science, 2008, 64: 1229-1236.

[12] 吴明根, 曹凤秋, 刘 亮. 磺酰脲类除草剂对抗、感性雨久花乙酰

乳酸合成酶活性的影响. 植物保护学报, 2007, 34(5): 545-548. Wu M G, Cao F Q, Liu L. Effect of sulfonylurea herbicides on the acetolactate synthases activity between resistant and susceptive of Monochoria korsakowii. Acta Phytophylacica Sinica, 2007, 34(5): 545-548. (in Chinese)

[13] 吴明根, 吴松权, 朴仁哲, 傅民杰, 曹凤秋. 磺酰脲类除草剂对抗

感性慈姑ALS活性的影响. 农药, 2007, 46(10): 701-703. Wu M G, Wu S Q, Piao R Z, Fu M J, Cao F Q. Effect of sulfonylurea herbicides on acetolactate synthase activity of resistant and susceptive biotypes of arrowwheed. Agrochemicals, 2007, 46(10): 701-703. (in Chinese)

[14] 许 贤, 王贵启, 张宏军, 樊翠芹, 李秉华, 苏立军, 王建平. 河北

省境内播娘蒿对苯磺隆抗药性研究初报. 西北农业学报, 2008, 17(2): 270-273.

Xu X, Wang G Q, Zhang H J, Fan C Q, Li B H, Su L J, Wang J P. Preliminary study on the resistance of Descurainia sophia to tribenuron-methyl in Hebei province. Acta Agriculturae Boreali-Occidentalis Sinica, 2008, 17(2): 270-273. (in Chinese) [15] Cui H L, Zhang C X, Zhang H J, Liu X, Liu Y, Wang G Q, Huang H J,

Wei S H. Confirmation of flixweed (Descurainia sophia) resistance to tribenuron in China. Weed Science, 2008, 56: 775-779.

[16] 唐贵章, 许永久, 梁龙坦, 王长征, 李古彬. 除草剂防除麦田猪殃

殃试验. 安徽农业科学, 2006, 34(8): 1627, 1653.

Tang G Z, Xu Y J, Liang L T, Wang C Z, Li G B. The test of herbicide control Galium aparine in wheat field. Journal of Anhui Agricultural Science, 2006, 34(8): 1627, 1653. (in Chinese)

[17] 王庆亚, 董立尧, 娄远来, 张守栋. 农田杂草抗药性及其检测鉴定

方法. 杂草科学, 2002, 2: 1-5.

Wang Q Y, Dong L Y, Lou Y L, Zhang S D. Rapid reliable test for screening resistance within weed. Weed Science, 2002, 2: 1-5. (in Chinese)

[18] Boutsalis P, Karotam J, Powles S B. Molecular basis of resistance to