CERVUS3.0说明书(8)

parentage analysis program整理自软件help文件,下载过的朋友可以直接在help里查看。

1. Cervus 3.0 uses essentially the same input file formats as Cervus 2.0. However Cervus

3.0 no longer reads space-delimited files. All input files must be delimited either by

commas (.csv) or by tabs (.txt). Any input file that does not have a suffix of .csv is

assumed to be tab-delimited text.

2. In Cervus 3.0 IDs, allele names and any other data in the input files may contain internal

space characters. There is no need to replace internal spaces with underscores. Leading and trailing spaces will be ignored as before.

3. Cervus 3.0 project files (.crv), allele frequency files (.alf) and simulation data files (.sim)

have new text-based formats. Files of these types generated by Cervus 2.0 cannot be read by Cervus 3.0. However the new formats are designed to be compatible with future

versions of Cervus. The allele frequency file format used by Cervus 3.0 is the same as the format used for user-defined allele frequencies in Cervus 2.0, while the project file and

simulation data files now use a standard ini file format.

4. By default Cervus 3.0 uses the revised likelihood equations of in

place of the original likelihood equations of that were used by

Cervus 2.0 (for comparison Cervus 3.0 offers the option to use the original likelihood

equations instead). With the revised equations more parentages are likely to be assigned overall. Users may also notice fewer parentages assigned with two or more mismatches than with the original likelihood equations. In order to reduce the number of parentages assigned with multiple mismatches, some users of Cervus 2.0 selected an artificially low value for the error rate in likelihood calculations. There is no need to follow this approach when using the revised likelihood equations in Cervus 3.0.

5. Cervus 3.0 offers the choice of LOD or Delta as the statistic used to assess the confidence

of parentage assignment to the most likely candidate parent. In most cases similar results will be obtained. However it is not possible to use Delta to assign single candidate parents in a parent pair analysis with unknown sexes. In this case, LOD should be used.

6. Cervus 3.0 does not offer the two-step parentage analysis available in Cervus 2.0. Instead

Cervus 3.0 offers the more powerful parent pair analysis, with or without known sexes. If the sexes of candidate parents are known it is not possible to list the candidate parent IDs