Patterns of Protein Synthesis and Tolerance of Anoxia in Roo(10)

Patterns of Protein Synthesis and Tolerance of Anoxia in Root

304Changetal.PlantPhysiol.Vol.122,2000

TableI.Summaryofdatafor48maizeseedlingroottipproteinsfrom48two-dimensionalPAGEgelspots

DifferencefromCalculatedMass

ProteinIdentified

Species

(GenBankAccessionNo.)%ofSequenceCovered

Theoretical/Observed

SpotIntensityRatioHypoxicNormoxic

SpotNo.MALDIMassa

PeptideSequencesMatchedb

Mr/kDpI

D

1

901.49995.541,083.621,186.601,258.601,274.591,325.571,340.651,421.731,437.721,502.801,874.012,209.012,877.412,893.41901.49995.531,083.611,186.601,212.611,258.601,274.591,309.581,325.571,340.661,421.741,437.731,502.791,874.012,144.012,209.002,356.262,877.442,893.47989.521,237.671,876.032,613.42

D0.44 0.010.000.000.00 0.01 0.01 0.02 0.01 0.010.000.00 0.06 0.07 0.070.00 0.02 0.010.000.040.00 0.01 0.01 0.01 0.010.010.00 0.010.00 0.04 0.07 0.01 0.04 0.010.010.040.020.07

(K)(I)(K)INPQAPLDK(V)(R)IIGVDLNPSR(F)(K)(T)(K)THPMNFLNER(T)

(K)THPMet-oxNFLNER(T)(K)SAESNMet-oxCDLLR(I)(R)KFGCTEFVNPK(D)(R)TDLPNVVELYMK(K)

(R)TDLPNVVELYMet-oxK(K)(K)FITHSVPFAEINK(A)

(K)(K)DHNKPVQEVLAEMTNGGVDR(S)

(K)AAVAWEAGKPLSIEEVEVAPPQAMEVR(V)

(K)AAVAWEAGKPLSIEEVEVAPPQAMet-oxEVR(V)1,004.54,1,212.66,1,290.60,2,010.00c(K)(I)(K)INPQAPLDK(V)(R)IIGVDLNPSR(F)(K)GTFFGNYKPR(T)(K)FGCTEFVNPK(D)(K)THPMNFLNER(T)

(K)THPMet-oxNFLNER(T)(K)SAESNMCDLLR(I)

(K)SAESNMet-oxCDLLR(I)(R)KFGCTEFVNPK(D)(R)TDLPNVVELYMK(K)

(R)TDLPNVVELYMet-oxK(K)(K)FITHSVPFAEINK(A)

(K)(K)ILFTSLCHTDVYFWEAK(G)

(K)DHNKPVQEVLAEMTNGGVDR(S)(K)VCVLSCGISTGLGASINVAKPPK(G)

(K)AAVAWEAGKPLSIEEVEVAPPQAMEVR(V)

(K)AAVAWEAGKPLSIEEVEVAPPQAMet-oxEVR(V)1,004.53,1290.60,1,783.87,2,010.02(K)ELLEWGSR(V)

(R)VSAANSRPPNPQ( )(R)ILHHTIGLPDFSQELR(C)

(R)ESKPVYLSISCNLPGLPHPTFSR(D)

881.28,1,188.65,1,589.85,1,742.96,2,051.09,2,308.82,2,820.40

( )AGFAGDDAPR(A)(R)AVFPSIVGRPR(H)(K)IWHHTFYNELR(V)

(K)SYELPDGQVITIGAER(F)(R)VAPEEHPVLLTEAPLNPK(A)

(R)TTGIVLDSGDGVSHTVPIYEGYALPHAILR(L)

873.03,891.00,945.57,1,066.10,1,132.55,1,443.71,1,459.71,1,531.77(K)EVAVFGCR(N)(K)YDTVHGQWK(H)(K)DAPMFVVGVNEK(E)

(K)DAPMet-oxFVVGVNEK(E)(R)AASFNIIPSSTGAAK(A)(R)VPTVDVSVVDLTVR(L)

ADH1Z.mays(X04049)42%

ConsistentwithWestern-blotresult

40.9/42

6.43/6.64

%d505.6

2

ADH1Z.mays(X04049)53%

ConsistentwithWestern-blotresult

40.9/416.43/6.64349.5

3

4

976.461,198.721,515.771,747.931,954.103,151.720.010.010.020.040.030.08

HomologoustoPDC2

O.sativa(U38199)9%

Inconclusivesearch

resultActinZ.mays(U60511)28%

64.3/645.90/6.18316.9

37.2/43.55.28/5.7195.5

5

951.481,133.541,305.671,321.661,434.781,498.870.010.000.020.010.020.02

GAPC3/4Z.mays

(U45856,U45857)

32%

36.4/35.57.02/6.75132.4