Patterns of Protein Synthesis and Tolerance of Anoxia in Roo(17)

Patterns of Protein Synthesis and Tolerance of Anoxia in Root

ProteinSynthesisandToleranceofAnoxia311

TableI.Continued

DifferencefromCalculatedMass

0.03

ProteinIdentified

Species

(GenBankAccessionNo.)%ofSequenceCoveredInconclusivesearchresult

Theoretical/Observed

SpotIntensityRatioHypoxicNormoxic

SpotNo.MALDIMass

PeptideSequencesMatched

Mr/kDpI

1,800.90

31

1,228.631,278.621,294.661,294.661,313.631,329.621,412.761,473.701,659.911,659.911,675.761,680.862,658.320.00 0.01 0.030.040.010.000.010.020.020.010.030.030.05

32

993.461,033.591,117.591,170.541,355.701,510.881,797.902,122.020.010.010.010.020.010.020.020.02

33

1,219.731,347.811,825.80

0.020.010.70

(K)IFDNMet-oxQKPAFVFDGR(N)

908.49,1,365.68,1,386.69,1,398.67,1,739.95,2,004.05,3,050.56(R)VEIIANDQGNR(T)(R)MVNHFVQEFK(R)(K)EIAEAYLGSTIK(N)

(R)Met-oxVNHFVQEFK(R)(R)FEELNMDLFR(K)

(R)FEELNMet-oxDLFR(K)(K)SSVHDVVLVGGSTR(I)(R)TTPSYVGFTDTER(L)

(R)QATKDAGVIAGLNVMet-oxR(I)(R)IINEPTAAAIAYGLDK(K)(K)ATAGDTHLGGEDFDNR(M)(K)NAVVTVPAYFNDSQR(Q)

(K)EQVFSTYSDNQPGVLIQVYEGER(A)1,197.69,1,390.63,1,426.78,1,437.75,1,487.72

(R)HAFGDQYR(A)(R)NILNGTVFR(E)(K)YFDLGLPHR(D)(K)SEGGYVWACK(N)(K)TIEAEAAHGTVTR(H)(R)LVPGWTKPICIGR(H)

(K)GGETSTNSIASIFAWTR(G)(R)DHYLNTEEFIDAVADELK(A)

855.06,1,034.58,1,319.78,1,378.68,1,484.72,1,495.84,1,607.82(K)(V)(K)(K)Heatshockprotein70

Z.mays(P11143)24%

70.6/725.22/5.6644.3

Homologoustoisocitratedehydrogenase

(NADP)N.tabacum(X77944)23%

46.7/476.06/6.5643.4

1389.990.30(K)1,318.710.01(R)34

744.39744.39890.521,039.671,119.501,401.711,424.632,116.052,132.012,257.082,674.262,931.340.000.020.010.01 0.010.02 0.05 0.01 0.05 0.12 0.12 0.15

(R)FFAFGR(V)(R)DDPKNR(S)(K)FSVSPVVR(V)(R)IRPVLTVNK(M)(K)EGALAEENMR(G)(R)GFVQFCYEPIK(Q)(R)LWGENFFDPATK(K)

(R)GHVFEEMQRPGTPLYNIK(A)

(R)GHVFEEMet-oxQRPGTPLYNIK(A)(K)STLTDSLVAAAGIIAQEVAGDVR(M)(R)ITDGALVVVDCIEGVCVQTETVLR(Q)(R)KGNDYLINLIDSPGHVDFSSEVTAALR(I)865.45,955.53,1,484.73,1,797.91,2,357.15

HomologoustoMDH

B.napus(X89451)

HomologoustoMDHprecursor

M.sativa(AF020271)

HomologoustoMDH

C.reinhardtii(U40212)

HomologoustoMDHglyoxysomal

precursorG.max(P37228)

Homologoustoelongationfactor2

B.vulgaris(Z97178)15%

ConsistentwithWestern-blotresult

35.7/35.59.18/6.6439.9

35.8/35.58.00/6.64

38.5/35.58.77/6.64

37.3/35.56.99/6.64

93.8/905.93/6.6339.4