Patterns of Protein Synthesis and Tolerance of Anoxia in Roo(19)

Patterns of Protein Synthesis and Tolerance of Anoxia in Root

ProteinSynthesisandToleranceofAnoxia313

TableI.Continued

DifferencefromCalculatedMass

0.060.060.060.030.130.100.15

ProteinIdentified

Species

(GenBankAccessionNo.)%ofSequenceCovered

Theoretical/Observed

SpotIntensityRatioHypoxicNormoxic

SpotNo.MALDIMass

PeptideSequencesMatched

Mr/kDpI

1,573.901,790.991,984.972,252.162,557.412,573.382,986.53

40

993.461,007.591,033.601,117.591,147.521,170.511,355.711,510.891,797.932,122.050.000.030.020.01 0.02 0.010.020.030.050.06

41

633.32678.36847.41939.46975.531,064.551,196.751,251.581,267.601,288.701,389.721,433.791,549.811,608.851,736.951,906.082,093.052,109.052,521.450.000.010.000.000.030.010.01 0.010.020.010.010.020.020.010.030.040.030.040.03

42

839.44989.551,152.651,152.651,244.661,283.741,501.731,636.832,124.112,140.082,292.152,292.153,019.74

0.000.010.050.020.020.020.030.070.080.060.02 0.050.09 0.010.01

43

734.451,021.56

(K)VNQIGSVTESIEAVK(M)

(R)AAVPSGASTGVYEALELR(D)(R)GNPTVEVDVFCSDGTFAR(A)(R)SGETEDTFIADLAVGLSTGQIK(T)(K)MTEEIGEQVQIVGDDLLVTNPTR(V)

(K)Met-oxTEEIGEQVQIVGDDLLVTNPTR(V)(K)SFVSEYPIVSIEDPFDQDDWVHYAK(M)742.18,877.05,893.02,1,658.89,1,716.91,1,830.03,2,268.2,2,384.06,2,807.44(R)HAFGDQYR(A)(K)WPLYLSTK(N)(R)NILNGTVFR(E)(K)YFDLGLPHR(D)(K)CATITPDEAR(V)(K)SEGGYVWACK(N)(K)TIEAEAAHGTVTR(H)(R)LVPGWTKPICIGR(H)

(K)GGETSTNSIASIFAWTR(G)(R)DHYLNTEEFIDAVADELK(A)

729.43,1,151.61,1,319.77,1,378.70,1,484.72,1,495.86,1,607.84,1,710.74(K)SIEER(A)(K)FGVEAR(A)(K)APGFGENR(K)(K)AIFTEGCK(S)(K)APGFGENRK(A)(K)LQTANFDQK(I)(K)IGVQIIQNALK(T)

(K)SVAAGMet-oxNAMDLR(R)

(K)SVAAGMet-oxNAMet-oxDLR(R)(R)(R)(A)

(R)GISMet-oxAVDAVVTNLK(S)(K)ELDKLQTANFDQK(I)(K)CELEDPLILIHDKK(V)(K)CELDDPLILIHEKK(I)

(K)TPVHTIASNAGVEGAVVVGK(L)(R)MISTSEEIAQVGTISANGER(E)

(R)Met-oxISTSEEIAQVGTISANGER(E)(K)QRPLLIVAEDVESEALGTLIINK(L)925.50,1,586.74,1,679.84,2,046.87,2,125.05(R)(E)(K)ASNPFVNLK(K)(K)LGTIDPYFVK(L)(K)LGTIDPYFVK(L)(K)CYIYLSGQVK(E)(K)DELDIVIPTIR(N)(K)(K)YIYTIDDDCFVAK(D)

(R)DLIGPAMet-oxYFGLMGDGQPIGR(Y)(R)DLIGPAMet-oxYFGLMet-oxGDGQPIGR(Y)

(K)NLLSPSTPFFFNTLYDPYR(E)(K)GIFWQEDIIPFFQNVTIPK(D)

( )Acet-AGTVTVPGSSTPSTPLLKDELDIVIPTIR(N)2,077.79,3,057.74(K)LLSVFR(E)

(K)SWLAFAAQK(V)

Homologoustoisocitrate

dehydrogenase(NADP )N.tabacum(X77944)27%

466.5631.8

Mitochondrialchaperonin60

Z.mays(L21006)35%

60.9/615.67/5.7329.4

Golgiassociatedproteinse-wap41

Z.mays(U89897)44%

41.2/38.55.75/6.0626.5

HomologoustoMetsynthase

84.9/776.10/6.1424.5