Patterns of Protein Synthesis and Tolerance of Anoxia in Roo(16)

Patterns of Protein Synthesis and Tolerance of Anoxia in Root

310Changetal.PlantPhysiol.Vol.122,2000

TableI.Continued

DifferencefromCalculatedMass

0.000.010.000.020.10

ProteinIdentified

Species

(GenBankAccessionNo.)%ofSequenceCovered

Theoretical/Observed

SpotIntensityRatioHypoxicNormoxic

SpotNo.MALDIMass

PeptideSequencesMatched

Mr/kDpI

1,130.581,130.581,658.831,991.022,296.33

26

684.40734.45958.531,041.571,096.601,096.601,470.801,470.801,658.861,699.951,807.021,807.021,864.931,991.062,296.31664.33734.40910.51931.41947.391,083.681,188.651,304.651,403.691,433.741,481.74

0.01 0.01 0.020.020.020.040.050.030.040.06 0.030.06 0.060.050.09 0.01 0.020.000.01 0.010.000.020.030.030.030.03

27

( )MASHIVGYPR(M)(R)IPSTEEIADR(I)

(K)YGAGIGPGVYDIHSPR(I)(K)LQEELDIDVLVHGEPER(N)

(K)LVVSTSCSLLHTAVDLVNEPK(L)

1,199.61,1,555.73,1,813.93,1,864.87,2,282.20,2,424.41(R)EGLPLR(K)(K)LLSVFR(E)

(R)GAKTLDLIK(G)

( )AcetN-ASHIVGYPR(M)(K)YLFAGVVDGR(N)(K)KISEDDYVK(A)

( )AcetN-ASHIVGYPRMet-oxGPK(R)(R)FETCYQIALAIK(D)

(K)YGAGIGPGVYDIHSPR(I)( )MASHIVGYPRMGPKR(E)(K)ILTALKGVTGFGFDLVR(G)

(K)GMet-oxLTGPVTILNWSFVR(N)(R)KYAEVKPALENMet-oxVSAAK(L)(K)LQEELDIDVLVHGEPER(N)

(K)LVVSTSCSLLHTAVDLVNEPK(L)

1,583.79,1,814.02,1,994.03,2,282.18(K)FDQVR(V)(R)IFAQGAK(L)(R)VHILTDGR(D)

(R)MYVTMet-oxDR(Y)

(R)Met-oxYVTMet-oxDR(Y)(R)LDQLQLLLK(G)(R)(T)(K)IYDGDGFNYIK(E)

(R)YAGMet-oxLQYDGELK(L)(R)GWDAQVLGEAPYK(F)(K)FGHVTFFWNGNR(S)

855.04,861.06,864.48,973.52,1,030.11,1,046.60,1,066.08,1,101.57,1,202.64,1,280.65,1,326.65,1,474.70,1,743.00,2,338.26,2,420.33,2,858.71(R)(Q)(K)SIVASGLAR(R)(K)(D)(K)TQVTVEYR(N)

(R)(K)TQVTVEYRNESGAR(V)(R)DDPDFTWEVVKPLK(W)(K)EHVIKPVIPEQYLDEK(T)

(K)IIIDTYGGWGAHGGGAFSGK(D)(K)ENFDFRPGMIIINLDLK(K)

(K)VLVNIEQQSPDIAQGVHGHFTK(R)(R)VHTVLISTQHDETVTNDEIAADLK(E)(K)TAAYGHFGRDDPDFTWEVVKPLK(W)832.31,1,141.63,1,414.72,1,874.93,2,206.08

(K)LAANAFLAQR(I)(K)AADLTYWESAAR(M)(K)FLNASVGFGGSCFQK(D)(K)IFDNMQKPAFVFDGR(N)

HomologoustoMetsynthase

C.roseus(X83499)21%

84.9/796.10/6.448.5

2,3-Bisphosphoglycerate-independentphosphoglyceratemutase

Z.maysM8091216%

60.6/635.29/648.2

2829

723.37873.51979.49995.531,453.801,609.761,688.901,937.051,963.992,035.102,417.352,649.442,649.440.000.000.010.010.04 0.030.050.010.030.030.090.110.13

Notidentified

HomologoustoS-adenosylMet

synthetaseO.sativa(Z26867)42%

N.A./3443.2/46N.A./5.745.74/6.0547.746.9

30

1,074.631,353.661,632.781,784.930.020.020.000.05HomologoustoUDP-Gldehydrogenase

G.max(U53418)10%

52.9/535.74/6.346.6