Patterns of Protein Synthesis and Tolerance of Anoxia in Roo(12)

Patterns of Protein Synthesis and Tolerance of Anoxia in Root

306Changetal.PlantPhysiol.Vol.122,2000

TableI.Continued

DifferencefromCalculatedMass 0.010.010.000.000.000.030.010.010.040.02 0.040.03 0.010.000.000.000.00 0.05

ProteinIdentified

Species

(GenBankAccessionNo.)%ofSequenceCoveredF1-ATPase, -subunit

Z.mays(M36087)32%

Theoretical/Observed

SpotIntensityRatioHypoxicNormoxic94.5

SpotNo.MALDIMass

PeptideSequencesMatched

Mr/kD59/55

pI6.01/5.63

11

11*

866.391,134.631,173.661,390.691,399.771,492.791,723.931,864.952,044.062,061.072,186.112,589.36702.41790.46839.511,052.541,312.762,197.98

12

675.32675.32719.38748.38765.42806.48900.45946.52978.52992.531,071.521,087.531,143.571,189.621,205.601,513.891,535.801,551.891,565.941,601.941,639.991,679.991,714.881,791.031,835.951,903.002,106.212,268.132,324.10951.481,133.551,305.671,321.661,434.771,498.871,775.832,033.09

0.00 0.02 0.010.020.030.030.00 0.020.020.000.000.020.000.020.000.010.040.030.040.090.090.030.040.100.070.070.100.010.060.000.010.020.010.010.020.030.02

13

(R)EGNDLYR(E)(K)TNHFLPIHR(E)(K)(G)

(K)AHGGFSVFAGVGER(T)(R)VGLTGLTVAEHFR(D)

(R)(Q)(R)LVLEVAQHLGENMet-oxVR(T)(R)DAEGQDVLLFIDNIFR(F)

(R)pyroGluISELGIYPAVDPLDSTSR(M)(R)QISELGIYPAVDPLDSTSR(M)(R)IPSAVGYQPTLATDLGGLQER(I)

(K)ITDEFTGAGAIGQVCQVIGAVVDVR(F)(K)SVIEVR(N)(K)(F)(K)AIGINVPR(S)(K)(K)VLQLETAAGAAIR(F)

(K)YSNSNIEIHTFNQSQYPR(I)965.543,1,274.63,1,520.79,1,661.82,2,438.33(K)TGAPCR(S)(R)APVEPY( )(K)ISGDSLK(D)

(R)pyro-GluIFDSR(G)(R)QIFDSR(G)(K)YNQLLR(I)(K)TYDLNFK(E)(K)TCNALLLK(V)(K)( )(K)ARQIFDSR(G)

(R)AGWGVMASHR(S)

(R)AGWGVMet-oxASHR(S)(K)DKTYDLNFK(E)(K)MGVEVYHNLK(S)

(K)Met-oxGVEVYHNLK(S)(K)LGANAILAVSLAVCK(A)(R)IEEELGDAAVYAGAK(F)(K)IPLYQHIANLAGNK(T)(K)AVSNVNNIIGPAIVGK(D)(K)VNQIGSVTESIEAVR(M)(K)VQIVGDDLLVTNPTR(V)(K)KIPLYQHIANLAGNK(T)

(K)VVIGMet-oxDVAASEFFGEK(D)(R)GAVPSGASTGIYEALELR(D)(R)(K)LAMet-oxQEFMet-oxILPTGASSFK(E)(K)EAMet-oxKMGVEVYHNLKSIIK(K)(R)SGETEDTFIADLSVGLSTGQIK(T)(K)YGQDATNVGDEGGFAPNIQENK(E)794.41,1,103.5,1,604.89,1,773.86(K)EVAVFGCR(N)(K)YDTVHGQWK(H)(K)DAPMFVVGVNEK(E)

(K)DAPMet-oxFVVGVNEK(E)(R)AASFNIIPSSTGAAK(A)(R)(L)(K)LVSWYDNEWGYSTR(V)

(K)FGIVEGLMet-oxTTVHAITATQK(T)

HomologoustoUDP-Glupyrophosphorylase

H.vulgare(X91347)13%

51.6/555.20/5.63

ENO1Z.mays(X55981)63%

48.1/525.20/5.8394.4

GAPC3/4Z.mays

(U45856,U45857)

32%

36.4/35.57.02/6.890.8