Patterns of Protein Synthesis and Tolerance of Anoxia in Roo(14)

Patterns of Protein Synthesis and Tolerance of Anoxia in Root

308Changetal.PlantPhysiol.Vol.122,2000

TableI.Continued

DifferencefromCalculatedMass0.000.000.000.01 0.010.01 0.030.00

ProteinIdentified

Species

(GenBankAccessionNo.)%ofSequenceCovered

Theoretical/Observed

SpotIntensityRatioHypoxicNormoxic

SpotNo.MALDIMass

PeptideSequencesMatched

Mr/kDpI

1,161.621,300.621,555.851,760.862,214.152,226.102,336.172,412.13

18

1,219.721,318.701,809.101,825.10873.48877.45877.45974.541,001.561,017.521,346.751,362.741,373.811,568.771,650.021,693.831,821.932,000.172,016.162,379.202,466.272,619.382,635.39

0.010.000.030.040.00 0.01 0.010.010.000.000.010.010.070.020.030.030.030.030.030.050.060.050.06

19

20

910.50915.391,083.671,087.551,167.521,188.641,304.621,323.681,387.661,403.651,433.711,481.711,557.741,589.832,311.172,420.242,434.252,629.542,721.27 0.01 0.02 0.010.00 0.010.010.010.050.00 0.010.000.010.010.020.000.070.020.020.04

(K)TAVANIPYGGAK(G)(K)DDGTLASYVGFR(V)(R)GVLFATEALLAEHGK(G)(K)GGIGCSPGDLSISELER(L)

(K)FHGYSPAVVTGKPVDLGGSLGR(D)(K)YIIEAANHPTDPEADEILSK(K)

(R)FVIQGFGNVGSWAAQLISEAGGK(V)(R)YHHEVDPDEVNALAQLMet-oxTWK(T)970.54,1,129.79,1,262.66,1,312.74,1,655.83,2,284.19(R)LFGVTTLDVVR(A)(R)DDLFNINAGIVK(S)

(K)VAILGAAGGIGQPLSLLMK(L)

(K)VAILGAAGGIGQPLSLLMet-oxK(L)1,347.82,1,861.96,2,316.21,2,656.37(R)ALGQISER(L)(K)NVTCLTR(L)(R)KEGMERK(D)(K)TSTGEKPVR(E)(R)LNVQVSDVK(N)(K)EFAPSIPEK(N)

(K)MELVDAAFPLLK(G)

(K)Met-oxELVDAAFPLLK(G)(K)(K)SQASALEAHAAPNCK(V)(K)VLVVANPANTNALILK(E)(K)(R)KFSSALSAASSACDHIR(D)

(R)VLVTGAAGQIGYALVPMIAR(G)

(R)VLVTGAAGQIGYALVPMet-oxIAR(G)(R)ELVSDDEWLNGEFITTVQQR(G)(K)NVIIWGNHSSSQYPDVNHATVK(T)

(K)GVVATTDVVEACTGVNVAVMVGGFPR(K)(K)GVVATTDVVEACTGVNVAVMet-oxVGGFPR(K)

886.51,1,277.71,2,032.14(R)VHILTDGR(D)(R)MYVTMDR(Y)(R)LDQLQLLLK(G)

(K)GVDAQIASGGGR(M)(R)YENDWDVVK(R)(R)YLVSPPEIDR(T)(K)IYDGDGFNYIK(E)(R)YENDWDVVKR(G)(R)YAGMLQYDGELK(L)

(R)YAGMet-oxLQYDGELK(L)(R)GWDAQVLGEAPYK(F)(K)FGHVTFFWNGNR(S)

( )AcetN-GSSGFSWTLPDHPK(L)(K)RGWDAQVLGEAPYK(F)

(K)ESFESGTLHLIGLLSDGGVHSR(L)(R)MVMLAKALEYADNFDRVR(V)(R)DVLDGSSIGFVETLENDLLELR(A)

(R)IQILTSHTLQPVPVAIGGPGLHPGVK(F)

(K)AHGTAVGLPSDDDMGNSEVGHNALGAGR(I)

HomologoustoMDHprecursor

M.sativa(AF020271)

12%

Inconclusivesearchresult

CytoplasmicMDH

Z.mays(AF007581)

39%

35.9/36.58.80/6.4465.3

35.6/36.55.93/6.1460.8

2,3-Bisphosphoglycerate-independentphosphoglyceratemutase

Z.mays(M80912)42%

60.6/645.29/5.8357.2