Patterns of Protein Synthesis and Tolerance of Anoxia in Roo(13)

Patterns of Protein Synthesis and Tolerance of Anoxia in Root

ProteinSynthesisandToleranceofAnoxia307

TableI.Continued

DifferencefromCalculatedMass 0.02

ProteinIdentified

Species

(GenBankAccessionNo.)%ofSequenceCovered

Theoretical/Observed

SpotIntensityRatioHypoxicNormoxic

SpotNo.MALDIMass

PeptideSequencesMatched

Mr/kDpI

2,200.02

14

910.501,087.561,188.651,304.631,475.671,557.781,589.802,434.322,629.60 0.010.010.020.010.030.05 0.010.080.08

15

16

675.33675.33748.36765.39806.45959.55978.511,071.521,087.521,551.851,601.851,639.901,679.981,698.881,714.841,790.931,835.891,886.971,902.942,106.162,268.082,324.052,968.39901.491,083.621,186.601,258.601,274.591,309.591,325.571,340.671,421.731,437.731,502.791,874.002,144.022,208.96845.53888.411,065.561,081.54

0.010.000.000.000.000.000.010.000.01 0.010.000.000.020.040.010.000.010.030.010.05 0.050.010.090.000.000.000.00 0.010.00 0.020.01 0.010.00 0.01 0.02 0.04 0.100.010.010.010.00

17

(K)GILGYVEEDLVSTDFQGDSR(S)

1,045.57,1,104.61,1,149.54,1,173.82,1,319.68,1,987.10(R)VHILTDGR(D)

(K)GVDAQIASGGGR(M)(R)YLVSPPEIDR(T)(K)IYDGDGFNYIK(E)(K)ALEYADFDNFDR(V)

( )AcetN-GSSGFSWTLPDHPK(L)(K)RGWDAQVLGEAPYK(F)

(R)DVLDGSSIGFVETLENDLLELR(A)(R)IQILTSHTLQPVPVAIGGPGLH-PGVK(F)

841.06,1,482.72,2,312.20,2,807.41(K)TGAPCR(S)(R)APVEPY( )

(R)pyro-GluIFDSR(G)(R)(G)(K)YNQLLR(I)

( )Ac-AVTITWVK(A)(K)( )

(R)AGWGVMASHR(S)

(R)AGWGVMet-oxASHR(S)(K)IPLYQHIANLAGNK(T)(K)VNQIGSVTESIEAVR(M)(K)VQIVGDDLLVTNPTR(V)(K)KIPLYQHIANLAGNK(T)(K)VVIGMDVAASEFFGEK(D)

(K)VVIGMet-oxDVAASEFFGEK(D)(R)GAVPSGASTGIYEALELR(D)(R)GNPTVEVDVGLSDGSYAR(G)(K)LAMQEFMet-oxILPTGASSFK(E)

(K)LAMet-oxQEFMet-oxILPTGASSFK(E)(K)EAMet-oxKMGVEVYHNLKSIIK(K)(R)SGETEDTFIADLSVGLSTGQIK(T)(K)YGQDATNVGDEGGFAPNIQENK(E)(K)SFVSEYPIESIEDPFDQDDWSTYAK(L)(K)GQTPVFPR(I)(R)IIGVDLNPSR(F)(K)GTFFGNYKPR(T)(K)THPMNFLNER(T)

(K)THPMet-oxNFLNER(T)(K)SAESNMCDLLR(I)

(K)SAESNMet-oxCDLLR(I)(R)KFGCTEFVNPK(D)(R)TDLPNVVELYMK(K)

(R)TDLPNVVELYMet-oxK(K)(K)FITHSVPFAEINK(A)

(K)GSTVAVFGLGAVGLAAAEGAR(I)(K)ILFTSLCHTDVYFWEAK(G)

(K)DHNKPVQEVLAEMTNGGVDR(S)1,987.08,1,993.98,2,010.02(K)SLLIPFR(E)(R)SHSCDLR(M)

(R)MGAFTLGVNR(V)

(R)Met-oxGAFTLGVNR(V)

2,3-Bisphosphoglycerate-independentphosphoglyceratemutase

Z.mays(M80912)23%

60.6/655.29/5.7687.0

ENO1Z.mays(X55981)60%

48.1/525.20/5.6885.6

ADH1Z.mays(X04049)37%

40.9/426.43/6.5467.3

Gludehydrogenase

Z.mays(D49475)40%

44.0/416.09/6.4265.6