Patterns of Protein Synthesis and Tolerance of Anoxia in Roo(11)

Patterns of Protein Synthesis and Tolerance of Anoxia in Root

ProteinSynthesisandToleranceofAnoxia305

TableI.Continued

DifferencefromCalculatedMass

0.030.030.000.010.010.020.010.070.060.060.040.030.11

ProteinIdentified

Species

(GenBankAccessionNo.)%ofSequenceCovered

Theoretical/Observed

SpotIntensityRatioHypoxicNormoxic

SpotNo.MALDIMass

PeptideSequencesMatched

Mr/kDpI

1,775.842,033.112,200.056

1,133.551,198.671,420.701,434.771,436.741,677.031,788.862,191.032,207.012,609.49

7

714.441,133.561,198.711,498.931,677.091,788.882,191.072,207.05807.41892.45972.41972.41984.50988.39988.391,078.571,094.531,126.541,830.94

0.010.020.050.080.120.080.080.07 0.03 0.020.02 0.02 0.020.00 0.030.00 0.04 0.04 0.01

8

9

901.491,083.631,186.621,212.591,258.621,274.621,309.611,325.601,340.691,421.761,437.751,502.831,874.051,219.721,318.701,809.101,825.10

0.000.010.020.010.020.020.020.020.020.030.020.030.030.010.000.030.04

10

(K)(K)FGIVEGLMet-oxTTVHAITATQK(T)(K)GILGYVEEDLVSTDFQGDSR(S)1,104.63,1,306.67,1,319.68(K)(K)AGIALNDHFIK(L)

(K)DAPMFVVGVNEDK(Y)(R)AASFNIIPSSTGAAK(A)

(K)DAPMet-oxFVVGVNEDK(Y)(K)TLLFGEKPVTVFGIR(N)(K)LVSWYDNEWGYSNR(V)

(K)GIMGYVEEDLVSTDFTGDSR(S)

(K)GIMet-oxGYVEEDLVSTDFTGDSR(S)(K)VIHDNFGIIEGLMTTVHAITATQK(T)

834.46,888.52,1,350.77,1,422.71,1,663.02,1,798.89,1,804.86,1,912.02,1,968.95,2,625.51(R)VVDLIR(H)

(K)YDTVHGQWK(H)(K)AGIALNDHFIK(L)

(R)VPTVDVSVVDLTVR(I)(K)TLLFGEKPVTVFGIR(N)(K)LVSWYDNEWGYSNR(V)

(K)GIMGYVEEDLVSTDFTGDSR(S)

(K)GIMet-oxGYVEEDLVSTDFTGDSR(S)1,663.05,1,800.91(R)LDYIQR(H)(R)FSISWPR(I)

(K)EMGMDAYR(F)(R)GDYPFSMR(S)(R)YGIVYVDR(N)

(K)Emet-oxGMDAYR(F)(R)GDYPFSMet-oxR(S)(R)IGLAFDVMGR(V)

(R)IGLAFDVMet-oxGR(V)(R)VPYGTSFLDK(Q)

(R)SWDINLGWFLEPVVR(G)

750.03,819.06,1,014.12,1,030.09,1,066.06,1,111.55,1,148.53,1,446.69,1,927.98(K)GQTPVFPR(I)(R)IIGVDLNPSR(F)(K)GTFFGNYKPR(T)(K)FGCTEFVNPK(D)(K)THPMNFLNER(T)

(K)THPMet-oxNFLNER(T)(K)SAESNMCDLLR(I)

(K)SAESNMet-oxCDLLR(I)(R)KFGCTEFVNPK(D)(R)TDLPNVVELYMK(K)

(R)TDLPNVVELYMet-oxK(K)(K)FITHSVPFAEINK(A)

(K)GSTVAVFGLGAVGLAAAEGAR(I)1,378.67

(R)LFGVTTLDVVR(A)(R)DDLFNINAGIVK(S)

(K)VAILGAAGGIGQPLSLLMK(L)

(K)VAILGAAGGIGQPLSLLMet-oxK(L)1,347.82,1,861.96,2,316.21,2,656.37

GAPC2Z.mays(U45858)35%

36.5/376.40/6.85113.6

GAPC2Z.mays(U45858)26%

36.5/376.40/6.65109.7

GLU1Z.mays(U44773)12%

64.2/606.23/6.12104.4

ADH1Z.mays(X04049)27%

40.9/436.43/6.5497.8

HomologoustoMDH

precursorM.sativa(AF020271)

12%

Inconclusivesearchresult

35.9/35.58.80/6.4397.4