Patterns of Protein Synthesis and Tolerance of Anoxia in Roo(15)

Patterns of Protein Synthesis and Tolerance of Anoxia in Root

ProteinSynthesisandToleranceofAnoxia309

TableI.Continued

DifferencefromCalculatedMass

ProteinIdentified

Species

(GenBankAccessionNo.)%ofSequenceCovered

Theoretical/Observed

SpotIntensityRatioHypoxicNormoxic

SpotNo.MALDIMass

PeptideSequencesMatched

Mr/kDpI

21

993.481,510.781,797.860.03 0.07 0.02

794.36,1,101.55,1,202.65,1,277.71,1,280.62,1,458.68,1,474.67,1,742.96,1,794.83,1,837.08,1,940.94,2,230.15,2,297.16,2,339.20

(R)HAFGDQYR(A)

(R)LVPGWTKPICIGR(H)

(K)GGETSTNSIASIFAWTR(G)1,149.58,1,378.57,2,108.01

22

622.28778.42914.58945.421,009.531,025.521,073.521,090.641,279.571,292.681,497.691,513.711,578.861,739.821,821.002,298.282,453.28 0.010.000.010.010.020.010.010.01 0.020.02 0.03 0.010.02 0.020.090.000.03

23

868.46888.46934.51954.441,300.601,388.751,950.932,234.910.000.010.000.000.010.01 0.01 0.05

24

790.45949.551,052.541,300.721,312.781,358.782,198.06 0.010.010.01 0.020.020.020.04

25

734.461,041.561,096.591,096.590.000.010.010.04

(R)NFEGR(V)(R)GTFANIR(I)(R)ILLESAIR(N)(K)DFNSYGSR(R)(K)LSVFDAAMR(Y)

(K)LSVFDAAMet-oxR(Y)(R)KDFNSYGSR(R)(R)VDKLPYSIR(I)

(R)DAMNKLGSDSNK(I)(K)FYSLPALNDPR(V)(R)SDETVAMIEAYLR(A)

(R)SDETVAMet-oxIEAYLR(A)(R)SNLVGMet-oxGIIPLCFK(S)(R)ATYESITKGNPMWNK(L)(R)RGNDEIMARGTFANIR(I)

(K)INPLVPVDLVIDHSVQVDVAR(S)(R)FDTEVELAYFNHGGILPYVIR(N)713.36,727.35,734.41,896.47,1,115.59,1,141.69,1,202.61,1,263.60,1,306.59,1,322.61,1,381.70,1,527.74,1,723.84,1,758.95,1,804.97,1,962.98,2,015.10,2,031.11,2,254.07,2,834.44(R)LFADFQK(R)(R)EISHQFK(V)

(K)VNVGVGAYR(D)(K)Nmet-oxGLYGQR(A)(K)TFTYYHPESR(G)

(R)IAAVQALSGTGACR(L)

(R)IFLEDGHQIGCAQSYAK(N)

(K)HFPFFDMet-oxAYQGFASGDPER(D)943.54,1,257.61,1,339.69,1,440.69,1,456.70,1,472.73,1,874.94(K)VANFLAR(F)(K)AGFISLVSR(Y)(K)GGTLISYEGR(V)(K)SIPSIVELDSLK(V)(K)VLQLETAAGAAIR(F)(R)IVTEDFLPLPSK(G)

(K)YSNSNIEIHTFNQSQYPR(I)

1,139.72,1,146.61,1,271.66,1,398.73,1,655.96,1,790.98,1,965.06(K)LLSVFR(E)

( )AcetN-ASHIVGYPR(M)(K)YLFAGVVDGR(N)(K)KISEDDYVK(A)

Homologoustoisocitratedehydrogenase

(NADP )N.tabacum(X77944)9%

InconclusivesearchresultHomologoustoaconitase

Arabidopsis(AC007170)

21%

46.7/446.06/6.4257.2

98.2/905.79/6.3454.6

HomologoustoAspaminotransferase

O.sativa(D67043)21%

47.5/407.64/6.5653.6

HomologoustoUDP-Glupyrophosphorylase

H.vulgare(X91347)17%

51.6/545.20/5.8352.7

HomologoustoMetsynthase

C.roseus(X83499)12%

84.9/786.10/6.4450.3